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Invitrogen™ Phosphate Sensor DFS Item

Phosphate Sensor

Manufacturer:  Invitrogen™ LSPV4406

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Catalog No. PV4406



Description

Description

The Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point mode. This product is a purified form of recombinant E. coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment. Binding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ~ 0.1μM), resulting in a large increase in fluorescence. The Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green. This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases.
Specifications

Specifications

Phosphate Sensor, 10nmol, 483μM Concentration, 430nm Excitation, 450nm Emission, MDCC Label, Fluorescent Detection Method, Kinetic Readout, Fluorescence Intensity Technique, Used For Pharma and Biopharma, Protein Assays and Analysis Target and Lead Identification and Validation
Phosphate Sensor
Liquid
10nmol
384-well plate
10nmol
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
–80°C
Documents

For Research Use Only. Not for use in diagnostic procedures.