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Invitrogen™ Phosphate Sensor 
Phosphate Sensor
$730.00 - $4500.00
Specifications
| For Use With (Equipment) | Microplate Reader |
|---|---|
| Target | GTPases, ATPases, Phosphatases, Phosphodiesterases |
| For Use With (Application) | Fluorescence Intensity |
| Assay | Phosphatase Assay |
| Content And Storage | Phosphate Sensor is supplied as a 500 μM solution in 10 mM Tris (pH 7.6) and 50 mM NaCl. Store product at -80°C. |
Description
The Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point mode. This product is a purified form of recombinant E. coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment. Binding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ∼ 0.1μM), resulting in a large increase in fluorescence. The Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green. This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases.Specifications
| Microplate Reader | |
| Fluorescence Intensity | |
| Phosphate Sensor is supplied as a 500 μM solution in 10 mM Tris (pH 7.6) and 50 mM NaCl. Store product at -80°C. | |
| MDCC | |
| 384-well plate | |
| Kinetic |
| GTPases, ATPases, Phosphatases, Phosphodiesterases | |
| Phosphatase Assay | |
| Fluorescent | |
| Phosphate Sensor | |
| 430⁄450 | |
| Dry Ice |
For Research Use Only. Not for use in diagnostic procedures.