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Invitrogen™ Phosphate Sensor
Manufacturer: Invitrogen™ LSPV4407
DescriptionThe Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point mode. This product is a purified form of recombinant E. coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment. Binding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ~ 0.1μM), resulting in a large increase in fluorescence. The Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green. This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases.
|Phosphate Sensor, 100nmol, 514μM Concentration, 430nm Excitation, 450nm Emission, MDCC Label, Fluorescent Detection Method, Kinetic Readout, Fluorescence Intensity Technique, Used For Pharma and Biopharma, Protein Assays and Analysis Target and Lead Identification and Validation|
|Pharma and biopharma, protein assays and analysis, target and lead identification and validation|
For Research Use Only. Not for use in diagnostic procedures.