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Thermo Scientific™ Phire Buffers

Choose from three formats of these buffers to optimize PCR using Thermo Scientific™ Phire™ Hot Start II DNA Polymerase.

Manufacturer:  Thermo Scientific™ F524L

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Catalog No. F524L




The reaction buffer for Thermo Scientific Phire Hot Start II DNA Polymerase is available in three formats: non-colored version with and without detergents or Green version containing a density reagent and two tracking dyes for direct loading of PCR products on gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

The concentration of Mg2+ in the reaction buffers is optimized to work well for most amplicons. The 5X buffer contains 1.5 mM MgCl2 at final reaction concentration. A separate tube of DMSO is provided for further optimization.


5x Phire Reaction Buffer, 50 mM MgCl2 solution, 100% DMSO.

Description & Specifications


Phire DNA Polymerases.
Store at -20°C. Stable for one year from the packaging date.
5x Phire Reaction Buffer, 50 mM MgCl2 solution, 100% DMSO.
4 x 1.5mL