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Thermo Scientific™ phi29 DNA Polymerase

Perform highly efficient isothermal DNA amplification with phi29 DNA Polymerase, a highly processive polymerase with strong strand displacement activity.

Manufacturer:  Thermo Scientific™ EP0094

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Catalog No. FEREP0094


 

Disclaimers:

Use of this enzyme in certain applications may be covered by patents and may require a license.


Specifications

Description

phi29 DNA Polymerase is a highly processive polymerase (up to more than 70 kb) featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification. phi29 DNA Polymerase also possesses a 3'→5' exonuclease (proofreading) activity acting preferentially on single-stranded DNA or RNA. Therefore 3'-modified primers are highly recommended.

Highlights

  • Highest processivity and strand displacement activity among known DNA polymerases – more than 70 kb long DNA stretches can be synthesized
  • Highly accurate DNA synthesis (see Reference 5)
  • Extremely high yields of amplified DNA even from minute amounts of template
  • Amplification products can be directly used in downstream applications (PCR, restriction digestion, SNP genotyping, etc.)
Description & Specifications

Specifications

phi29 DNA Polymerase
Rolling circle amplification (RCA) (6): generation of periodic DNA nanotemplates (7); Multiple displacement amplification (MDA) (8); Unbiased amplification of whole genome (WGA) Addition of Pyrophosphatase to the reaction mixture with phi29 DNA Polymerase
0.1ug/uL, (10ug/uL), 50ug (5000U)
5 x 1mL of 10X Reaction Buffer
50μg (5000U)