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Thermo Scientific™ phi29 DNA Polymerase


Perform highly efficient isothermal DNA amplification with phi29 DNA Polymerase, a highly processive polymerase with strong strand displacement activity.

Manufacturer: thermo scientific™  EP0092

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 Disclaimers

Use of this enzyme in certain applications may be covered by patents and may require a license.

Catalog No. FEREP0092

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Description & Specifications

Specifications

For Use With (Application) Rolling circle amplification (RCA) (6): generation of periodic DNA nanotemplates (7); Multiple displacement amplification (MDA) (8); Unbiased amplification of whole genome (WGA) Addition of Pyrophosphatase to the reaction mixture with phi29 DNA Polymerase
Includes 1mL of 10X Reaction Buffer
Product Name phi29 DNA Polymerase
Quantity 10μg (1000U)
Item Description 0.1ug/uL, (10ug/uL), 10ug (1000U)

phi29 DNA Polymerase is a highly processive polymerase (up to more than 70 kb) featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification. phi29 DNA Polymerase also possesses a 3'→5' exonuclease (proofreading) activity acting preferentially on single-stranded DNA or RNA. Therefore 3'-modified primers are highly recommended.

Highlights

  • Highest processivity and strand displacement activity among known DNA polymerases – more than 70 kb long DNA stretches can be synthesized
  • Highly accurate DNA synthesis (see Reference 5)
  • Extremely high yields of amplified DNA even from minute amounts of template
  • Amplification products can be directly used in downstream applications (PCR, restriction digestion, SNP genotyping, etc.)