Thermo Scientific™ phi29 DNA Polymerase
Perform highly efficient isothermal DNA amplification with phi29 DNA Polymerase, a highly processive polymerase with strong strand displacement activity.
Manufacturer: Thermo Scientific™ EP0091
phi29 DNA Polymerase is a highly processive polymerase (up to more than 70 kb) featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification. phi29 DNA Polymerase also possesses a 3'→5' exonuclease (proofreading) activity acting preferentially on single-stranded DNA or RNA. Therefore 3'-modified primers are highly recommended.
- Highest processivity and strand displacement activity among known DNA polymerases – more than 70 kb long DNA stretches can be synthesized
- Highly accurate DNA synthesis (see Reference 5)
- Extremely high yields of amplified DNA even from minute amounts of template
- Amplification products can be directly used in downstream applications (PCR, restriction digestion, SNP genotyping, etc.)
|phi29 DNA Polymerase|
|Rolling circle amplification (RCA) (6): generation of periodic DNA nanotemplates (7); Multiple displacement amplification (MDA) (8); Unbiased amplification of whole genome (WGA) Addition of Pyrophosphatase to the reaction mixture with phi29 DNA Polymerase|
|1mL of 10X Reaction Buffer|
|0.1ug/uL, (10ug/uL), 2.5ug (250U)|