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Thermo Scientific™ Pierce™ Staphylococcus aureus V-8 Protease and Kt


Specifically cleave only at glutamic acid residues or cleave at both glutamic and aspartic residues with our immobilized Staphylococcus aureus V-8 protease kit.

Manufacturer: thermo scientific™  PI20151

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Catalog No. PI20151

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Description & Specifications

Specifications

Description Pierce Staphylococcus aureus V-8 Protease Kit
Formulation

Multi-component kit with agarose resin (activity 35-60 units/mL), buffers and accessories

Kit Contents

Resin Separators, 6

MS-Compatible No
Quantity 2.5mL resin kit
Sufficient For 20 digestions of 2mg protein samples
Storage Requirements 4°C

Thermo Scientific™ Pierce V-8 Protease Kit contains &italic_begin;Staphylococcus aureus&italic_end; V-8 protease immobilized on resin and three digestion buffers for specific cleavage at glutamic acid residues or cleavage at glutamic and aspartic acid residues.

Has two pH optima, one at pH 4.0 and the other at pH 7.8 and is specific for cleavage at the carboxy terminus of glutamic acid and aspartic acid residues. The protease is further specific for only glutamic digestion in buffers, which do not contain phosphate at either pH optimum. Phosphate buffers at pH 7.8 will facilitate digestion at both glutamic and aspartic acid residues.

Highlights:

  • Specificity for glutamic acid is achieved in ammonium bicarbonate, pH 7.8 or ammonium acetate, pH 4
  • Enzyme is active in the presence of many denaturing agents such as SDS, urea and guanidine·HCl