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Thermo Scientific™ PageRuler™ Unstained Protein Ladder


A mixture of seven native proteins (14.4 to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Manufacturer: thermo scientific™  26614

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Catalog No. PI26614

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Description & Specifications

Specifications

Description PageRuler Unstained Protein Ladder
Detection Visible
Formulation 14 proteins from 10 to 200kDa in reducing sample buffer
Quantity 2 × 250mL
Separation Range Mid
Stained Unstained
Sufficient For 50 to 100 mini gel lanes at 5 to 10μL per lane

Thermo Scientific™ Unstained Protein Molecular Weight Marker is a mixture of seven native proteins (14.4 to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Each protein in the ladder contains an integral Strep-tag™ II Sequence which can be detected directly on Western blots using a Strep-Tactin™ Conjugates or an antibody against Strep-tag™ II Sequence. The Ladder produces sharp bands on SDS-polyacrylamide gel following staining of the gel with Thermo Scientific Pierce Gel Stains or other coomassie dye or silver stains. The 50kDa protein band is of greater intensity and serves as a reference band.

Highlights:

  • Size range – 14 proteins spanning 10 to 200kDa

  • Ready-to-use – supplied in a loading buffer for direct loading on gels

  • Sharp bands – excellent accuracy for detection in with coomassie or silver stains

  • Reference band – 50kDa band is has greater intensity for easy orientation

  • Tagged – each protein in the ladder contains an integral Strep-tag™ II Sequence

  • Quality tested – each lot evaluated by SDS-PAGE and Western blotting

Includes:

Proteins (0.02 to 0.05mg/mL of each) in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 100mM DTT, 1mM NaN3, 0.01% bromophenol blue and 33% glycerol.

Recommended for:

Accurate protein sizing on SDS-polyacrylamide gels and Western blots