Thermo Scientific™ PageRuler™ Unstained Low Range Protein Ladder
A mixture of seven purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small protein gel electrophoresis.
Manufacturer: Thermo Scientific™ 26632
Thermo Scientific™ PageRuler Unstained Low Range Protein Ladder is a mixture of seven (7) purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small protein gel electrophoresis.The protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 100kDa protein band indicates the approximate position of large proteins and the 25kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins (except for the 5 and 3.4kDa peptides) contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained Broad Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.
Size range – seven proteins and one peptide spanning 3.4 to 100kDa
Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil
Sharp bands – excellent accuracy for detection in with coomassie or silver stains
Reference bands – 25kDa band is stronger than the others for easy orientation
Tagged – each protein in the ladder contains an integral Strep-tag™ II Sequence
Quality tested – each lot evaluated by SDS-PAGE and Western blotting
Proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 100mM DTT, 1mM NaN3, 0.01% bromophenol blue and 33% glycerol.
Accurate protein sizing on SDS-polyacrylamide gels and Western blots
|PageRuler Unstained Low Range Protein Ladder|
|8 peptides and proteins from 3.4 to 100kDa in reducing sample buffer|
|50 to 100 mini gel lanes at 5 to 10μL per lane|
|2 × 250mL|