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Thermo Scientific™ PageRuler™ Prestained NIR Protein Ladder


A mixture of 10 proteins (11 to 250KDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing following electrophoresis.

Manufacturer: thermo scientific™  26635

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Catalog No. PI26635

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Description & Specifications

Specifications

Description PageRuler Prestained NIR Protein Ladder
Detection Fluorescent, Visible
Formulation 10 proteins from 11 to 250kDa in reducing sample buffer
Quantity 2 × 250mL
Separation Range Broad
Stained Prestained
Sufficient For 50 to 250 mini gel lanes at 1 to 5μL per lane for infrared imaging detection

The Thermo Scientific™ PageRuler Prestained NIR Protein Ladder is a mixture of 10 proteins (11 to 250KDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing following electrophoresis.

The protein MW markers in this ladder resolve into sharp bands when analyzed by SDS-PAGE and are labeled with a fluorescent dye for visualization with instruments equipped for detection of near-infrared (NIR) fluorescence. The bands are also directly visible because the proteins are prestained blue. The 55kDa band is of greater intensity and serves as a reference band. The protein ladder is conveniently packaged and ready to use: no heating, dilution or addition of a reducing agent is required before use.

Highlights:

  • Size range 10 proteins spanning 11 to 250kDa

  • Fluorescent detect using 670nm red laser or 700nm channels with fluorescence imagers

  • Ready-to-use supplied in a loading buffer for direct loading on gels

  • Sharp bands excellent sizing for near-infrared visualization of protein gels and blots

  • Reference band 55kDa band has greater intensity for easy orientation

  • Quality tested each lot evaluated by SDS-PAGE and Western blotting

Includes:

Proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol

Requires:

Fluorescent imager capable of near-infrared fluorescence detection, such as certain Typhoon™ Imagers and the LI-COR Odyssey™ Infrared Imaging System

Recommended for:

Protein sizing on SDS-polyacrylamide gels and Western blots for visualization with near-infrared (NIR) fluorescence imagers