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Thermo Scientific™ PageRuler™ Prestained NIR Protein Ladder


A mixture of 10 proteins (11 to 250KDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing following electrophoresis.

Manufacturer: thermo scientific™  26635

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Catalog No. PI26635

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Description & Specifications

Specifications

Quantity 2 × 250mL
Sufficient For 50 to 250 mini gel lanes at 1 to 5μL per lane for infrared imaging detection
Separation Range Broad
Stained Prestained
Description PageRuler Prestained NIR Protein Ladder
Detection Fluorescent, Visible
Formulation 10 proteins from 11 to 250kDa in reducing sample buffer
Range Broad

The Thermo Scientific™ PageRuler Prestained NIR Protein Ladder is a mixture of 10 proteins (11 to 250KDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing following electrophoresis.

The protein MW markers in this ladder resolve into sharp bands when analyzed by SDS-PAGE and are labeled with a fluorescent dye for visualization with instruments equipped for detection of near-infrared (NIR) fluorescence. The bands are also directly visible because the proteins are prestained blue. The 55kDa band is of greater intensity and serves as a reference band. The protein ladder is conveniently packaged and ready to use: no heating, dilution or addition of a reducing agent is required before use.

Highlights:

  • Size range 10 proteins spanning 11 to 250kDa

  • Fluorescent detect using 670nm red laser or 700nm channels with fluorescence imagers

  • Ready-to-use supplied in a loading buffer for direct loading on gels

  • Sharp bands excellent sizing for near-infrared visualization of protein gels and blots

  • Reference band 55kDa band has greater intensity for easy orientation

  • Quality tested each lot evaluated by SDS-PAGE and Western blotting

Includes:

Proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol

Requires:

Fluorescent imager capable of near-infrared fluorescence detection, such as certain Typhoon™ Imagers and the LI-COR Odyssey™ Infrared Imaging System

Recommended for:

Protein sizing on SDS-polyacrylamide gels and Western blots for visualization with near-infrared (NIR) fluorescence imagers