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Thermo Scientific™ PageRuler™ Prestained 10-180kDa Protein Ladder


Mixture of 10 blue-, orange- and green-stained proteins (10 to 180kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Manufacturer: thermo scientific™  26616

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Catalog No. PI26616

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Description & Specifications

Specifications

Description PageRuler™ Prestained Protein Ladder, 2 x 250mL, 10 to 180kDa, Store at –20°C, Prestained Stain Type, Protein Biology, Protein Gel Electrophoresis Application, Two Reference Bands, Membrane-Compatible
Kit Contents Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol
Stain Type Prestained
Storage Requirements –20°C
Product Type PageRuler™ Prestained Protein Ladder
Quantity 2 × 250mL
Quantity 2 x 250μL
For Use With (Application) Protein biology, protein gel electrophoresis, monitoring protein migration during SDS-polyacrylamide gel electrophoresis, monitoring protein transfer onto membranes after western blotting, Sizing of proteins on SDS-polyacrylamide gels and western blots
Sufficient For 50 to 100 mini gel lanes at 5 to 10μL per lane
Stained Prestained
Detection Visible
Formulation 10 proteins from 10 to 170kDa in reducing sample buffer
Separation Range Mid

Thermo Scientific™ PageRuler Prestained Protein Ladder is a mixture of ten (10) blue-, orange- and green-stained proteins (10 to 180kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. The ladder contains one orange reference band at 70kDa and one green band at 10kDa.

Highlights:

  • Size range – 10 proteins spanning 10 to 180kDa

  • Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil

  • Sharp bands – color-coded bands of similar intensity for easy visualization

  • Quality tested – each lot evaluated by SDS-PAGE and Western blotting

  • Two reference bands – orange at 70kDa and green at 10kDa

  • Membrane-compatible – colored bands transfer to membranes for Western blotting

Includes:

Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.

Recommended for:

Monitoring protein migration during SDS-polyacrylamide gel electrophoresis; Monitoring protein transfer onto membranes after Western blotting; Sizing of proteins on SDS-polyacrylamide gels and Western blots