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OPTIZYME™ XbaI, Fisher BioReagents™

$128.27 - $370.92

Specifications

Cut Site T.CTAGA
Includes 10X OPTIZYME™ Buffer 4
Incubator Temperature 37°C
pH 7.4
Storage Buffer 10mM Tris HCl (pH 7.4), 100mM KCl, 7mM 2-mercaptoethano, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
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Products
Catalog Number Mfr. No. Concentration Quantity Units Price Quantity    

BP8008-1

 
fisher scientific company
BP8008-1
10U/μL 2000U 2,000U Each for $128.27

BP8008-5

 
fisher scientific company
BP8008-5
50U/μL 10000U 10,000U Each for $370.92
Description & Specifications

Specifications

Cut Site T.CTAGA
Includes 10X OPTIZYME™ Buffer 4
Incubator Temperature 37°C
pH 7.4
Storage Buffer 10mM Tris HCl (pH 7.4), 100mM KCl, 7mM 2-mercaptoethano, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol

5'...T^C T A G A...3'
3'...A G A T C^T...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity

  • 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 50 - 100%
  • Buffer 2: 50 - 100%
  • Buffer 3: 20 - 50%
  • Buffer 4: 100%
  • Buffer 5: 0 - 20%
Storage Buffer:
10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 7mM 2-mercaptoethanol, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
Ligation and Re-cleavage:
More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with XbaI.
Digestion of Agarose-embedded DNA:
A minimum 5 units of XbaI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Notes:
  • XbaI is blocked by overlapping dam methylation.
  • To avoid dam methylation, use a dam-, dcm- strain.
Compatible Ends: BcuI, Eco130I, NheI, XmaJI
Methylation Effects:
Dam: May overlap - blocked
  • 5'...TCTAGm6A TC...3'
  • 3'...AGATC Tm6AG...5'
  • (Cleavage is blocked)
Dcm: Never overlaps - no effect
CpG: Never overlaps - no effect
EcoKI: Never overlaps - no effect
EcoBI: Never overlaps - no effect