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OPTIZYME™ T4 Polynucleotide Kinase, Fisher BioReagents™

$288.75 - $288.75

Specifications

Color Colorless
Product Name T4 Polynucleotide Kinase
For Use With (Application) Probes for hybridization or transcript mapping, primers for PCR or DNA sequencing and markers for Gel electrophoresis, removal of 3'-phosphate groups
pH 7.5
Source E. coli cells with a cloned pse T gene of bacteriophage T4
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Products
Catalog Number Mfr. No. Quantity Price Quantity    

BP80981

 
fisher scientific company
BP8098-1
500U Each for $288.75
Description & Specifications

Specifications

Color Colorless
Product Name T4 Polynucleotide Kinase
For Use With (Application) Probes for hybridization or transcript mapping, primers for PCR or DNA sequencing and markers for Gel electrophoresis, removal of 3'-phosphate groups
pH 7.5
Source E. coli cells with a cloned pse T gene of bacteriophage T4
Storage Buffer 10X OPTIZYME™ T4 PNK Buffer 1 (for forward reaction): 500mM Tris HCl (7.6 pH at 25°C), 100mM MgCl2, 50mM DTT, 1mM spermidine, 10X OPTIZYME™ T4 PNK Buffer 2 (for exchange reaction): 500mM imidazole-HCl
Storage Requirements -20°C

OPTIZYME™ T4 PNK catalyzes the transfer of γ-phosphate from ATP to the 5'-OH group of DNA, RNA, oligonucleotides and nucleoside 3'-monophosphates.

Applications and Features:

  • OPTIZYME™ T4 PNK labels 5'-termini of nucleic acids (for use as: probes for hybridization or transcript mapping, primers for PCR or DNA sequencing, and markers for gel-electrophoresis)
  • 5'-phosphorylation of oligonucleotides, PCR products, and other DNA or RNA prior to ligation
  • Phosphorylation of PCR primers
  • Removal of 3'-phosphate groups
  • Active in buffers for OPTIZYME restriction enzymes, T4 DNA Ligase and Reverse Transcriptases

Supplied With:

  • 10X OPTIZYME T4 PNK Buffer 1 (for forward reaction): 500mM Tris-HCl (pH7.6 at 25°C), 100mM MgCl2, 50mM DTT, 1mM spermidine
  • 10X OPTIZYME T4 PNK Buffer 2 (for exchange reaction): 500mM imidazole-HCl (pH6.4 at 25°C), 180mM MgCl2, 50mM DTT, 1mM spermidine, 1mM ADP
  • 24% PEG 6000 Sol for T4 PNK: 24% (w/v) polyethylene glycol 6000
Source: E. Coli cells with a cloned pse T gene of bacteriophage T4
Quality Control:
  • Endo-, exodeoxyribonuclease and ribonuclease-free
  • Functionally tested for labeling of 5'-termini of DNA