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OPTIZYME™ T4 DNA Polymerase, Fisher BioReagents™

$504.06 - $504.06

Specifications

Color Colorless
Concentration 5U/μL
Product Name T4 DNA Polymerase
Format Liquid
For Use With (Application) Synthesis of labeled DNA probes, creation of blunt PCR products with 3'-dA overhangs, ligation-independent cloning of PCR products, Restriction Enzymes, reverse transcriptases and T4 DNA ligase
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Catalog Number Mfr. No. Quantity Price Quantity    

BP81055

 
fisher scientific company
BP81055
500U Each for $504.06
Description & Specifications

Specifications

Color Colorless
Concentration 5U/μL
Product Name T4 DNA Polymerase
Format Liquid
For Use With (Application) Synthesis of labeled DNA probes, creation of blunt PCR products with 3'-dA overhangs, ligation-independent cloning of PCR products, Restriction Enzymes, reverse transcriptases and T4 DNA ligase
pH 7.5
Source E. coli cells with a cloned gene 43 of bacteriophage T4
Storage Buffer OPTIZYME™ T4 DNA Pol Buffer 5X: Tris HCl 335mM (8.8 pH at 25°C), MgCl2 33mM, DTT 5mM, (NH4)2SO4 84mM
Storage Requirements -20°C

OPTIZYME™ T4 DNA Pol is a template-dependent DNA polymerase that catalyzes 5'->3' synthesis from primed single-stranded DNA.

Applications and features:

  • OPTIZYME T4 DNA Polymerase creates blunt DNA ends by filling in 5'-overhangs and/or removing 3'-overhangs
  • Creation of blunt PCR products with 3'-dA overhangs
  • Oligonucleotide-directed site-specific mutagenesis
  • Synthesis of labeled DNA probes (by replacement reaction)
  • Ligation-independent cloning of PCR products
  • Active in buffers for OPTIZYME restriction enzymes, reverse transcriptases and T4 DNA ligase
  • 3'->5' exonuclease activity
  • Lacks 5'->3' exonuclease activity

Supplied with:
5X OPTIZYME T4 DNA Pol Buffer: 335mM Tris-HCl (pH 8.8 at 25°C), 33mM MgCl2, 5mM DTT, 84mM (NH4)2SO4

Source: E. Coli cells with a cloned gene 43 of bacteriophage T4

Quality Control:
Endodeoxyribonuclease-free