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OPTIZYME™ T4 DNA Ligase, Fisher BioReagents™

Manufacturer: fisher scientific company  BP8099-5

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Catalog No. BP80995


Description & Specifications


Product Name T4 DNA Ligase
Format Liquid
Concentration 5U/μL
Color Colorless
Source E. coli cells with a cloned gene 30 of bacteriophage T4
pH 7.5
Quantity 500U
For Use With (Application) Used to clone restriction Enzyme generated DNA fragments, cloning PCR products, ligation of double-stranded Oligonucleotide linkers or adaptors to DNA, site-directed mutagenesis, amplified fragment length polymorphism, ligase-mediated RNA detection, nick
Storage Buffer OPTIZYME™ T4 DNA Ligase Buffer 10X: Tris HCl 400mM, MgCl2 100mM, DTT 100mM, ATP 5mM (7.8 pH at 25°C), OPTIZYME™ PEG 4000 Solution 50%: Polyethylene Glycol 50% (w/v)
Storage Requirements -20°C

OPTIZYME™ T4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Applications and Features:

  • OPTIZYME™ T4 DNA Ligase is used to clone restriction enzyme generated DNA fragments
  • Fast sticky end ligation (10min at room temperature)
  • Cloning PCR products
  • Ligation of double-stranded oligonucleotide linkers or adaptors to DNA
  • Site-directed mutagenesis
  • Amplified fragment length polymorphism
  • Ligase-mediated RNA detection
  • Nick repair in duplex DNA, RNA or DNA/RNA hybrids
  • Active in buffers for OPTIZYME restriction enzymes

Supplied with:

  • 10X OPTIZYME T4 DNA Ligase Buffer : 400mM Tris-HCl, 100mM MgCl2, 100mM DTT, 5mM ATP (pH7.8 at 25°C)
  • 50% OPTIZYME PEG 4000 Solution: 50% (w/v) polyethylene glycol
Source: E. Coli cells with a cloned gene 30 of bacteriophage T4
Quality control:
  • Endo-, exodeoxyribonuclease, phosphatase and ribonuclease-free
  • Functionally tested for ligation of cohesive and blunt end DNA fragments