missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

OPTIZYME™ T4 DNA Ligase, Fisher BioReagents™

$100.12 - $659.80

Specifications

Color Colorless
Product Name T4 DNA Ligase
For Use With (Application) Used to clone restriction Enzyme generated DNA fragments, cloning PCR products, ligation of double-stranded Oligonucleotide linkers or adaptors to DNA, site-directed mutagenesis, amplified fragment length polymorphism, ligase-mediated RNA detection, nick
pH 7.5
Source E. coli cells with a cloned gene 30 of bacteriophage T4
View More Specs


Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
BP80991 Fisher Scientific Company
BP80991
100U Each for $100.12
BP80995 Fisher Scientific Company
BP80995
500U Each for $659.80
Specifications

Description

OPTIZYME™ T4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Applications and Features:

  • OPTIZYME™ T4 DNA Ligase is used to clone restriction enzyme generated DNA fragments
  • Fast sticky end ligation (10min at room temperature)
  • Cloning PCR products
  • Ligation of double-stranded oligonucleotide linkers or adaptors to DNA
  • Site-directed mutagenesis
  • Amplified fragment length polymorphism
  • Ligase-mediated RNA detection
  • Nick repair in duplex DNA, RNA or DNA/RNA hybrids
  • Active in buffers for OPTIZYME restriction enzymes

Supplied with:

  • 10X OPTIZYME T4 DNA Ligase Buffer : 400mM Tris-HCl, 100mM MgCl2, 100mM DTT, 5mM ATP (pH7.8 at 25°C)
  • 50% OPTIZYME PEG 4000 Solution: 50% (w/v) polyethylene glycol
Source: E. Coli cells with a cloned gene 30 of bacteriophage T4
Quality control:
  • Endo-, exodeoxyribonuclease, phosphatase and ribonuclease-free
  • Functionally tested for ligation of cohesive and blunt end DNA fragments

Description & Specifications

Specifications

Colorless
Used to clone restriction Enzyme generated DNA fragments, cloning PCR products, ligation of double-stranded Oligonucleotide linkers or adaptors to DNA, site-directed mutagenesis, amplified fragment length polymorphism, ligase-mediated RNA detection, nick
E. coli cells with a cloned gene 30 of bacteriophage T4
-20°C
T4 DNA Ligase
7.5
OPTIZYME™ T4 DNA Ligase Buffer 10X: Tris HCl 400mM, MgCl2 100mM, DTT 100mM, ATP 5mM (7.8 pH at 25°C), OPTIZYME™ PEG 4000 Solution 50%: Polyethylene Glycol 50% (w/v)