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OPTIZYME™ PvuII, Fisher BioReagents™

Manufacturer: fisher scientific company  BP8022-5

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Catalog No. BP8022-5

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Description & Specifications


Product Name PvuII
Format Liquid
Concentration 10U/μL
Cut Site CAG.CTG
Incubator Temperature 37°C
pH 7.4
Includes >50% Glycerin, 25 to 50% Water
Quantity 5000U
Units 5,000U
For Use With (Application) >90% of DNA fragments can be ligated and re-cut after 10-fold over-digestion with Pvu II
Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Storage Requirements Keep container tightly closed

5'...C A G^C T G...3'
3'...G T C^G A C...5'

Supplied with: 10X OPTIZYME Buffer 2
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 2: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 50 - 100%™
  • Buffer 2: 100%
  • Buffer 3: 20 - 50%
  • Buffer 4: 20 - 50%™
  • Buffer 5: 50 - 100%
™ Star activity appears at a greater than 5-fold over-digestion
Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 90% of the DNA fragments can be ligated, and more than 95% of these can be re-cut, after 10-fold over-digestion with PvuII.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of PvuII is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Note: Greater than 15-fold over-digestion with PvuII may result in star activity.
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Never overlaps - no effect
  • EcoKI: Never overlaps - no effect
  • EcoBI: May overlap - effect not determined