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OPTIZYME™ NcoI, Fisher BioReagents™

Manufacturer: fisher scientific company  BP8017-1

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Catalog No. BP8017-1


Description & Specifications


Product Name NcoI
Format Liquid
Concentration 10U/μL
Cut Site C.CATGG
pH 7.4
Includes 25 to 50% Water, >50% Glycerin
Quantity 500U
Units 500U
For Use With (Application) >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Nco I
Storage Buffer 10mM Tris HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Storage Requirements Keep container tightly closed

5'...C^C A T G G...3'
3'...G G T A C^C...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 20 - 50%
  • Buffer 2: 20 - 50%
  • Buffer 3: 20 - 50%
  • Buffer 4: 100%
  • Buffer 5: 50 - 100%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with NcoI.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of NcoI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Note: Low salt, high glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.
  • Compatible Ends: AflIII, PscI, BtgI, Eco130I, FatI, PagI
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Never overlaps - no effect
  • EcoKI: Never overlaps - no effect
  • EcoBI: Never overlaps - no effect