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OPTIZYME™ HindIII, Fisher BioReagents™

Manufacturer: fisher scientific company  BP8006-1

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Catalog No. BP8006-1

  • / Each

Description & Specifications


Product Name HindIII
Format Liquid
Concentration 10U/μL
Cut Site A.AGCTT
Incubator Temperature 37°C
Includes 25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride
Quantity 7500U
Units 7,500U
For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII
Storage Buffer 10mM Tris HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Storage Requirements Keep container tightly closed

5'...A^A G C T T...3'
3'...T T C G A^A...5'

Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 - 20%
  • Buffer 2: 20 - 50%
  • Buffer 3: 0 - 20%
  • Buffer 4: 50 - 100%
  • Buffer 5: 100%
Storage Buffer:
10mM Tris-HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
Ligation and Re-cleavage:
More than 95% of the DNA fragments can be ligated and recut after a 50-fold over-digestion with HindIII.
Digestion of Agarose-embedded DNA:
A minimum 5 units of HindIII is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Methylation Effects:
Dam: Never overlaps - no effect
Dcm: Never overlaps - no effect
CpG: Never overlaps - no effect
EcoKI: Never overlaps - no effect
EcoBI: May overlap - cleavage impaired
  • 5'...TGm6AAGCTT(N)3 TGCT...3'
  • 3'...AC TTCGAA(N)3m6ACGA...5'
  • (Cleavage is impaired)