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OPTIZYME™ DraI, Fisher BioReagents™

Manufacturer:  Fisher BioReagents BP80261

Catalog No. BP8026-1



Description

Description

5'...T T T^A A A...3'
3'...A A A^T T T...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 50 - 100%
  • Buffer 2: 50 - 100%
  • Buffer 3: 20 - 50%
  • Buffer 4: 100%
  • Buffer 5: 20 - 50%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with DraI.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of DraI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Never overlaps - no effect
  • EcoKI: May overlap - blocked
    • 5'...TTTAAm6AC(N)6G TGC...3'
  • 3'...AAATT TG(N)6Cm6ACG...5'
  • (Cleavage is blocked)
  • EcoBI: Never overlaps - no effect
  • Specifications

    Specifications

    DraI
    10U/μL
    10X OPTIZYME™ Buffer 4
    7.4
    >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with DraI
    Keep container tightly closed
    Liquid
    TTT.AAA
    37°C
    2,000U
    10mM Tris HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
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