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OPTIZYME™ DdeI (HpyF31), Fisher BioReagents™


Manufacturer: fisher scientific company  BP8060-1

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Catalog No. BP8060-1

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Description & Specifications

Specifications

Product Name Dde I
Format Liquid
Concentration 10U/μL
Cut Site C.TNAG
Incubator Temperature 37°C
pH 7.4
Includes 25 to 50% Water, >50% Glycerin
Quantity 500U
For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI
Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Storage Requirements Keep container tightly closed

5'...C^T N A G...3'
3'...G A N T^C...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
  • Incubate at 37°C
  • Enzyme Activity in OPTIZYME buffers:
    • Buffer 1: 20 - 50%
    • Buffer 2: 20 - 50%
    • Buffer 3: 20 - 50%
    • Buffer 4: 100%
    • Buffer 5: 20 - 50%

    Storage Buffer:
    • 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycero
    l
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI.
  • Compatible Ends: BbvCI, Bpu10I, Bpu1102I, Eco81I
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Never overlaps - no effect
  • EcoKI: Never overlaps - no effect
  • EcoBI: May overlap - effect not determined