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OPTIZYME™ Csp6I (CviQI), Fisher BioReagents™

Manufacturer:  Fisher BioReagents BP80681

Catalog No. BP8068-1



Description

Description

5'...G^T A C...3'
3'...C A T^G...5'

Supplied with: 10X OPTIZYME Buffer 1
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 1: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl)2 and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 100%
  • Buffer 2: 50 - 100%
  • Buffer 3: 0 - 20%
  • Buffer 4: 50 - 100%
  • Buffer 5: 0 - 20%

Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with Csp6I.
  • Compatible Ends: FspBI, NdeI, Tru1I, VspI
  • Methylation Effects:
  • Dam: never overlaps - no effect
  • Dcm: never overlaps - no effect
  • CpG: may overlap - no effect
    • 5'...m5C GTAm5C G...3'
  • 3'... Gm5CAT Gm5C...5'
  • (No effect on cleavage)
  • EcoKI: never overlaps - no effect
  • EcoBI: never overlaps - no effect
  • Specifications

    Specifications

    Csp6 I
    10U/μL
    25 to 50% Water, >50% Glycerin
    7.4
    >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Csp6I
    Keep container tightly closed
    Liquid
    G.TAC
    37°C
    1500U
    10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
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