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OPTIZYME™ Alkaline Phosphatase, Fisher BioReagents™


Manufacturer: fisher scientific company  BP8097-1

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Catalog No. BP80971

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Description & Specifications

Specifications

Product Name Alkaline Phosphatase
Format Solid
Concentration 1U/μL
Color Colorless
Source E. coli cells with a cloned bacterial Alkaline Phosphatase gene
pH 7.4
Quantity 1000U
For Use With (Application) DePhosphorylates cloning vector DNA (10 min. at 37°C), fast thermo-inactivation (5 min. at 75°C), Nucleotide degradation prior to sequencing PCR products, active on Nucleotides, Protein dephosphorylation, restriction En
Storage Buffer OPTIZYME™ AP Buffer 10X : Tris HCl 100mM (8 pH at 37°C), MgCl2 50mM, KCl 1M, Triton™ X-100 0.2%, BSA 1mg/mL
Storage Requirements -20°C

OPTIZYME™ AP is a novel alkaline phosphatase that catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA and nucleotides in 10min.

Applications and Features:

  • OPTIZYME™ Alkaline Phosphatase dephosphorylates cloning vector DNA (10min at 37°C)
  • Fast thermoinactivation (5min at 75°C)
  • Simultaneous digestion and dephosphorylation of vector DNA
  • Single protocol for all types of DNA ends
  • Nucleotide degradation prior to sequencing PCR products
  • Active on nucleotides
  • Protein dephosphorylation
  • Active in all OPTIZYME restriction enzyme buffers

Supplied with:
10X OPTIZYME AP Buffer: 100mM Tris-HCl (pH 8.0 at 37°C), 50mM MgCl2, 1M KCl, 0.2% (v/v) Triton X-100, 1mg/mL BSA
Source: E. Coli cells with a cloned bacterial alkaline phosphatase gene
Quality Control:

  • Endo-, exodeoxyribonuclease and ribonuclease-free
  • Functionally tested for dephosphorylation of 5'-termini of overhanging, recessed and blunt DNA