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OCT3/4 Rat anti-Human, Mouse, Alexa Fluor 488, Clone: EM92, eBioscience™

Rat Monoclonal Antibody

$216.05 - $506.05

Specifications

Antigen OCT3/4
Clone EM92
Host Species Rat
Gene Alias OCT3, OCT4, octamer-binding transcription factor, POU5F1
Species Reactivity Human, Mouse
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 Disclaimers

For Research Use Only.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

5016773

 
life technologies
LS53584180
25μg Each for $216.05

5016774

 
life technologies
LS53584182
100μg Each for $506.05
Description & Specifications

Specifications

Antigen OCT3/4
Clone EM92
Host Species Rat
Gene Alias OCT3, OCT4, octamer-binding transcription factor, POU5F1
Species Reactivity Human, Mouse
Applications Flow Cytometry (Intracellular Staining)
Applications Immunocytochemistry
Regulatory Status RUO
Conjugate Alexa Fluor 488
Format Conjugated
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 0.5mg/mL

The EM92 monoclonal antibody reacts with mouse and human Oct3/4, encoded by the Pou5F1 gene. Oct3/4 is a POU domain-containing transcription factor that is critical for maintaining embryonic stem (ES) and induced pluripotent stem (iPS) cells in a pluripotent state, and is expressed by ES, embryonic germ cells and embryonic carcinoma cell lines. In cells of the inner cell mass (ICM), reduction of Oct3/4 expression causes dedifferentiation to trophoectoderm, whereas increased expression results in differentiation to mesoderm and primitive endoderm. Oct3/4 regulates the expression of several genes, including FGF-4, UTF1, Sox2, Fbx15, Rex1 and osteopontin through distinct mechanisms. Furthermore, Oct3/4 frequently acts synergistically with Sox2 to regulate target gene expression, as is the case with FGF-4. It has been demonstrated that Oct3/4 expression in ES cells can be negatively regulated by either treatment with retinoic acid, or by removal of leukemia-inhibitory factor (LIF).