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Nur77 Mouse anti-Mouse, Alexa Fluor® 488, Clone: 12.14, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS53596582

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Catalog No. 501128996



Description

Description

Description: This 12.14 monoclonal antibody reacts with mouse Nur77 (also known as NR4A1, TR3, NGFI-B, or NAK1), an inducible orphan nuclear receptor. Expressed in thymocytes and T cell lines, Nur77 promotes apoptosis and plays a role in thymocyte negative selection. Additionally, Nur77 has been shown to be critical for steroid biosynthesis in Leydig cells as well as for the effects of dopamine. In addition, Nur77 has been shown to interact with FoxP3 in regulatory T cells. However, our results with this antibody do not correlate with this observation. Applications Reported: This 12.14 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 12.14 antibody has been tested by intracellular staining followed by flow cytometric analysis of stimulated mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. NGFI-B-alpha is expressed in brain, adrenal, thyroid, liver, testis, ovary, thymus, muscle, lung and prostate. Its expression is induced in response to various stress stimuli and growth factors. The encoded protein acts as a nuclear transcription factor involved in mediating apoptotic signaling in thymocytes and tumor cells, as well as having a role in signaling in the hypothalamic-pituitary axis. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Three transcript variants encoding two distinct isoforms have been identified for this NR4A1. Variant (3), also known as TRC beta, contains multiple differences compared to variant 1. The resulting isoform b contains a shorter and distinct C-terminus compared to isoform a. RXR has been shown to be a partner for NR4A1.
Specifications

Specifications

Nur77
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P12813
early response protein NAK1; GFRP1; growth factor-inducible nuclear protein N10; HMR; hormone receptor; N10; NAK-1; nerve growth factor IB nuclear receptor variant 1; NGFIB; NP10; nuclear hormone receptor NUR/77; nuclear protein N10; nuclear receptor subfamily 4 group A member 1; nuclear receptor subfamily 4, group A, member 1; orphan nucle; orphan nuclear receptor HMR; orphan nuclear receptor TR3; ST-59; steroid receptor TR3; testicular receptor 3; TR3; TR3 orphan receptor
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
12.14
Alexa Fluor® 488
Nr4a1
Liquid
Nr4a1
IgG1
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
15370
Documents

For Research Use Only.