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Invitrogen™ Novex TNF- Rat ELISA Kit DFS Item

Designed to detect and quantify the level of Rat TNF-α in serum, plasma, and supernatant using 96-well plates and a microplate reader

Manufacturer: invitrogen™  KRC3012

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Catalog No. KRC3012


Description & Specifications


For Use With (Application) Colorimetric
Gene ID (Entrez) 24835
Includes Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.
Precision <4pg/mL
Quantity 192 assays
Regulatory Status RUO - research use only
Sample Type Plasma, Serum, Supernatants
Sample Size 50μL
Test Time 2.75 hrs
Target Tumor necrosis factor

  • The total assay incubation time is only 4 hours
  • A monoclonal capture antibody specific for Rat TNF-α has been coated onto the wells of the 96-well plate provided
  • Samples, including a standard of known Rat TNF-α content, controls, and unknowns, are pipetted into these wells
  • During the first incubation, the Rt TNF-α antigen binds to the immobilized (capture) antibody on one site
  • After washing, a biotinylated monoclonal antibody specific for Rt TNF-α is added
  • During the second incubation, this antibody binds to the immobilized Rt TNF-α captured during the first incubation
  • After washing, a streptavidin-horseradish peroxidase (HRP) is added
  • This binds to the biotinylated detection antibody to complete the four–member sandwich
  • After a second incubation and washing to remove all unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color
  • The intensity of this colored product is directly proportional to the concentration of Rat
  • TNF-α present in the original specimen and the optical density can be read on a standard microplate reader

Convenient Kit Format
The kit comes with a capture antibody precoated to the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips allowing you to run as few samples as you want. Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency.

Apoptosis, Apoptosis Related Factors, Cell Analysis, Cell Signaling, Cell Viability, Proliferation and Function, Immunoassays, Ready-To-Use Immunoassay

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