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Invitrogen™ Novex TNF- Mouse ELISA Kit DFS Item

Designed to detect and quantify the level of mouse TNF-α in serum, plasma, and supernatant using 96-well plates and a microplate reader

$525.00 - $2,089.50

Specifications

For Use With (Application) Colorimetric
Gene ID (Entrez) 21926
Precision <3pg/mL
Regulatory Status RUO - research use only
Sample Size 50μL
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Includes:  Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

KMC3011

DFS Item
 
invitrogen™
KMC3011
96 assays Each for $525.00

KMC3012

DFS Item
 
invitrogen™
KMC3012
192 assays Each for $980.70

KMC3011C

DFS Item
 
invitrogen™
KMC3011C
480 assays Each for $2,089.50
Description & Specifications

Specifications

For Use With (Application) Colorimetric
Gene ID (Entrez) 21926
Precision <3pg/mL
Regulatory Status RUO - research use only
Sample Size 50μL
Sample Type Plasma, Serum, Supernatants
Target Tumor necrosis factor
Test Time 2.5 hrs

  • The total assay incubation time is only 2.5 hour
  • A monoclonal capture antibody specific for Mouse TNF-α has been coated onto the wells of the 96-well plate provided
  • Samples, including a standard of known Mouse TNF-α content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinylated polyclonal detector antibody
  • During the first incubation, Mouse TNF-α antigen binds to the immobilized capture antibody and to the solution phase biotinylated detector antibody on a second site
  • After washing, a streptavidin-horseradish peroxidase (HRP) is added
  • This binds to the biotinylated detection antibody to complete the four–member sandwich
  • After a second incubation and washing to remove all unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color
  • The intensity of this colored product is directly proportional to the concentration of Mouse TNF-α present in the original specimen and the optical density can be read on a standard microplate reader

The kit comes with a capture antibody precoated to the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips allowing you to run as few samples as you want. Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency.

Apoptosis, Apoptosis Related Factors, Cell Analysis, Cell Signaling, Cell Viability, Proliferation and Function, Immunoassays, Ready-To-Use Immunoassay

Shipping Condition: Wet ice

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