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Invitrogen™ Novex™ Ni-NTA Agarose DFS Item


Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence

Manufacturer: invitrogen™  R90115

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Catalog No. R90115

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Description & Specifications

Specifications

Kit Contents The Ni-NTA resin is pre-charged and able to bind up to 50mg of recombinant protein per 1mL of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+
Quantity 25mL

  • Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine
  • One-step purification can be performed under both native and denaturing conditions
  • Ni-NTA uses the chelating ligand nitrilotriacetic acid (NTA) coupled to a cross-linked 6% agarose resin that is suitable for use in batch and gravity flow applications

Applications:

His-Tagged Protein Purification, Protein and Peptide Purification, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis