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Invitrogen™ ELISA Stop Solution DFS Item

A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution.

Supplier:  Invitrogen™ SS04

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Catalog No. SS04



Description

Description

  • A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution
  • As TMB substrate solution reacts with horseradish peroxidase, it produces a pronounced blue color
  • At the end of TMB incubation, a volume of ELISA Stop Solution equal to the volume of TMB substrate solution is added to the wells to terminate the horseradish peroxidase reaction
  • Addition of Stop Solution results in the rapid formation of a yellow end product
  • The optical density of this yellow end product is measured at 450 nm
  • The yellow end product is stable for sixty minutes after addition of Stop Solution, optical density should be measured within this time period
  • Store at 2°C to 8°C
Specifications

Specifications

ELISA Stop Solution
Liquid
Store at 2°C to 8°C
1 L
HRP, TMB
Wet Ice
SDS
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For Research Use Only. Not for use in diagnostic procedures.