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Invitrogen™ Novex IL-6 Mouse ELISA Kit DFS Item

To detect and quantify the level of mouse IL-6 in serum, plasma, and supernatant using 96-well plates and a microplate reader

Manufacturer: invitrogen™  KMC0061C

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Catalog No. KMC0061C


Description & Specifications


For Use With (Application) Colorimetric
Gene ID (Entrez) 16193
Includes Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.
Precision <3pg/mL
Quantity 480 assays
Regulatory Status RUO - research use only
Sample Type Plasma, Serum, Supernatants
Sample Size 50μL, 100μL supernatant
Test Time 3.5 hrs
Target Interleukin 6

  • Easy to run
  • Rapid protocol
  • Convenient precoated, removable 8-well strips
  • Easy-to-Run Sandwich ELISA
  • The total assay incubation time is only 3.5 hours.
  • A monoclonal capture antibody specific for Mouse IL-6 has been coated onto the wells of the 96-well plate provided
  • Samples, including standard of known Mouse IL-6 content, controls, and°Cs, are pipetted into these wells followed by an addition of a biotinylated polyclonal detector antibody
  • During the first incubation, Mouse IL-6 antigen binds to the immobilized capture antibody and to solution phase biotinylated detector antibody on second site
  • After washing, streptavidin-horseradish peroxidase (HRP) is added
  • This binds to the biotinylated detection antibody to complete four-member sandwich
  • After second incubation and washing to remove all unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color
  • The intensity of this colored product is directly proportional to the concentration of Mouse IL-6 present in the original specimen and the optical density can be read on a standard microplate reader

Convenient Kit Format:The kit comes with a capture antibody precoated to the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips allowing you to run as few samples as you want. Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency.

Cell Analysis, Immunoassays, Ready-To-Use Immunoassay

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