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Invitrogen™ Novex IL-2 Mouse ELISA Kit DFS Item


Designed to detect and quantify the level of mouse IL-2 in serum, plasma, and supernatant using 96-well plates and a microplate reader

Manufacturer: invitrogen™  KMC0022

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Catalog No. KMC0022

  • / Each
 

Description & Specifications

Specifications

For Use With (Application) Colorimetric
Gene ID (Entrez) 16183
Includes Pre-coated plate, standard, controls, detector antibody, Streptavidin-HRP, diluents, wash buffer, incubation buffer, chromogen, stop solution, and plate covers.
Precision <8pg/mL
Quantity 192 assays
Regulatory Status RUO - research use only
Sample Type Plasma, Serum, Supernatants
Sample Size 50μL
Test Time 3 hrs
Target Interleukin 2

Easy to run
Rapid protocol
Convenient precoated, removable 8-well strips

Easy-to-Run Sandwich ELISA
The total assay incubation time is only 3 hours. A monoclonal capture antibody specific for Mouse IL-2 has been coated onto the wells of the 96-well plate provided. Samples, including a standard of known Mouse IL-2 content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinylated polyclonal detector antibody. During the first incubation, Mouse IL-2 antigen binds simultaneously to the immobilized capture antibody and to the solution phase biotinylated detector antibody on a second site. After washing, a streptavidin-horseradish peroxidase (HRP) is added. This binds to the biotinylated detection antibody to complete the four–member sandwich. After a second incubation and washing to remove all unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Mouse IL-2 present in the original specimen and the optical density can be read on a standard microplate reader.

Ready-to-Use Mouse IL-2 ELISA Kit Contains These Reagents, Buffers, and Supplies:
• Mouse IL-2 Antibody Coated 96-Well Plate
• Mouse IL-2 Standard
• Standard Diluent Buffer
• Mouse IL-2 Detection Antibody
• Streptavidin-HRP
• HRP Diluent
• Wash Buffer Concentrate (25X)
• Stabilized Chromogen, TMB
• Stop Solution
• Plate Covers
• Detailed protocol with validation tests

Convenient Kit Format
The kit comes with a capture antibody precoated to the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips allowing you to run as few samples as you want.

Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency.

Cell Analysis, Immunoassays, Ready-To-Use Immunoassay

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