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Invitrogen™ Novex™ IFN-γ Human ELISA Kit DFS Item


Each ready-to-use kit ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency.

Manufacturer: invitrogen™  KHC4021

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Catalog No. KHC4021

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Description & Specifications

Specifications

Accession Number NM_000619.2, NP_000610.2
For Use With (Application) Colorimetric Detection
Gene ID (Entrez) 3458
Includes Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.
Incubation Time 2.75 hr.
For Use With (Equipment) Microplate Reader
Marker Gene IFN-γ
Quantity 96 assays
Sensitivity <4pg/mL
Sample Type Serum, Supernatants
Sample Size 50μL
Target interferon, gamma

Detects and quantifies the level of human IFN-γ in serum and supernatant using 96-well plates and a microplate reader. Convenient precoated, 96-well plate supplied as removable 8-well strips to accommodate smaller samples run. Easy-to-run sandwich ELISA has a total assay incubation time is only 2.75 hours. A monoclonal capture antibody specific for Human IFN-γ machine coated onto the wells of the 96-well plate, ensuring low well-to-well variability and eliminating the need for overnight coating. Samples, including a standard of known Human IFN-γ content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinylated polyclonal detector antibody. During the first incubation, Human IFN-γ antigen binds simultaneously to the immobilized capture antibody and to the solution phase biotinylated detector antibody on a second site. After washing, a streptavidin-horseradish peroxidase (HRP) is added. This binds to the biotinylated detection antibody to complete the four–member sandwich. After a second incubation and washing to remove all unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Human IFN-γ present in the original specimen and the optical density can be read on a standard microplate reader.

See protocol insert for more information on validation.

Cell Analysis, Cell Signaling, Cell Viability, Proliferation & Function, Immunoassays, Ready-To-Use Immunoassay

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