missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

Invitrogen™ Novex™ Caspase-3 Colorimetric Protease Assay Kit DFS Item

Simple and convenient means for quantitating caspases that recognize amino acid sequence, DEVD

$341.00 - $854.00


Conjugate pNA
Gene ID (Entrez) 836
View More Specs


For Research Use Only. Not for use in diagnostic procedures.

Includes: Cell Lysis Buffer (25mL): Tris buffered saline (TBS) -containing detergent; 2X Reaction Buffer (2mL): contains buffered saline, glycerol and detergent; Substrate (125μL): contains 4mM of synthetic peptide (sequence: DEVD) conjugated to the chromophore pNA in DMSO; Dilution Buffer (25mL): buffered solution containing HEPES, Tris and NaCl; DTT (100μL): contains 1 M dithiothreitol

Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
KHZ0021 Invitrogen™
25 tests Each for $341.00
KHZ0022 Invitrogen™
200 tests Each for $854.00


The Caspase-3 colorimetric protease assay includes substrate and optimized buffers. The substrate, DEVD-pNA, is composed of the chromophore p-nitroanilide (pNA), and a synthetic tetrapeptide, DEVD (Asp-Glue-Val-Asp), which is the upstream amino acid sequence of the Caspase-3 cleavage site in PARP. Upon cleavage of the substrate by Caspase-3 or related caspases, free pNA light absorbance can be quantified using a spectrophotometer or a microplate reader at 400 or 405 nm. Comparison of the absorbance of pNA from apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-3 activity.

  • Includes 96-well plate, cuvettes

Apoptosis, Apoptosis Related Factors, Caspase Activation, Cell Analysis, Cell Viability, Proliferation and Function, Enzyme and Protein Activity Assays, Protease/Peptidase Activity, Proteins, Expression, Isolation and Analysis

Order Info

Shipping condition: Dry ice

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Description & Specifications