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Novex™ AcTEV™ Protease DFS Item

Specifically recognizes a seven amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly), ideal for removing affinity tags from fusion proteins

Manufacturer: life technologies  12575015

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Catalog No. 12575015

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Description & Specifications


Includes AcTEV™ Protease is supplied with a vial of 20X TEV buffer [1M Tris-HCl (pH 8.0), 10mM EDTA] and a vial of 100mM DTT.
Product Type Protease
Storage Requirements Store at -20°C. Guaranteed stable for 6 months when properly stored.
Quantity 1000 units

An improved version of Tobacco Etch Virus (TEV) AcTEV™ Protease is highly site-specific, highly active and significantly more stable than native TEV protease, resulting in enhanced long-term activity.

  • Highly specific cleavage activity
  • Enhanced enzyme stability for prolonged protease activity
  • Activity over a broad temperature (4°C to 30°C) and pH (6.0 to 8.5) range
  • Six-histidine sequence to facilitate its removal from the digested protein sample
  • Greater than 85% single-band purity with no nonspecific protease contamination

  • Application
    • Incubation with AcTEV™ Protease releases the protein of interest from the fusion tag
    • This is an effective way to remove solubility, secretion, detection, and purification tags from recombinant protein
    Enzyme specifications
    • Purified from E. coli expressing the AcTEV™ Protease gene
  • Unit definition
    • One unit of AcTEV™ Protease cleaves 85% of a 3μg control substrate in 1 hr at 30°C
  • Unit reaction conditions
    • 50mM Tris-HCl (pH 8.0), 0.5mM EDTA, 1mM DTT, 3μg control substrate, and 1 unit enzyme in 30μL for 1 hr at 30°C AcTEV™ Protease is functionally tested for the absence of any nonspecific protease activity

    GST-Tagged Protein Purification, His-Tagged Protein Purification, Protein and Peptide Purification, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis