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Invitrogen™ Novex™ Aβ40 Human ELISA Kit DFS Item


Quantifies natural and recombinant human Aβ40 (Beta Amyloid 40) selectively in tissue culture medium, tissue homogenate, and cerebrospinal fluid (CSF)

Manufacturer: invitrogen™  KHB3481

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Catalog No. KHB3481

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Description & Specifications

Specifications

Conjugate HRP (Horseradish Peroxidase)
Concentration 9.8-500pg/mL
Gene ID (Entrez) 351
Incubation Time 4 hr.
Marker Gene Aβ 40
Quantity 96 assays
Sensitivity <6pg/mL
Sample Type Cerebrospinal Fluid, Tissue Homogenates
Sample Volume 50μL
Species Human
Target amyloid beta (A4) precursor protein

Easy-to-run sandwich ELISA
The Aβ40 ELISA kit is a solid phase sandwich enzyme-linked immuno-sorbent assay (ELISA). A monoclonal antibody specific for the NH2-terminus of human Aβ has been coated onto the wells of the microtiter strips provided. During the first incubation, standards of known human Aβ40 content, controls, and unknown samples are pipetted into the wells and co-incubated with a rabbit antibody specific for the COOH-terminus of the 1–40 Aβ sequence. Bound rabbit antibody is detected through use of a horseradish peroxidase-labeled anti-rabbit antibody. Samples are quantitated using a standard curve generated from the recombinant Aβ40 included with the kit.

Results typically in one day—rapid 4-hour incubation protocol
The kit comes with the capture antibody precoated to the wells, eliminating the need to coat the plate. No overnight incubation needed. The incubation steps can be performed in four hours.

Precision: <10% CV inter-assay and intra-assay
The kits are validated for precision to help ensure that results are consistent within a plate, between plates, and between lots.

Economical, removable wells
The precoated wells come as removable 8-well strips, allowing you to tailor the assay size to your needs. Two standard vials are provided to give you the option of reconstituting a new vial should you choose to not run the entire plate at once.

  • Easy-to-run sandwich ELISA
  • Results typically in one day—rapid four-hour incubation protocol
  • Precision: <10% CV intra-assay and inter-assay
  • Precoated, removable 8-well strips
  • Rigorous validation
  • Each ELISA kit is validated for sensitivity, specificity, and lot-to-lot consistency

This kit does not detect Aβ42 or Aβ43. See protocol insert for more information on validation.

Cell Analysis, Immunoassays, Ready-To-Use Immunoassay

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