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Thermo Scientific™ North2South™ Chemiluminescent Hybridization and Detection Kit


Acquire consistent, highly sensitive Southern blot results with a novel ECL HRP substrate and optimized hybridization and blocking buffers.

Manufacturer: thermo scientific™  17175

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Catalog No. PI-17175

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Description & Specifications

Specifications

For Use With (Application) Non-radioactive Southern blotting
Formulation Multi-component kit
Includes Stabilized Streptavidin-Horseradish Peroxidase Conjugated, 1.5mL
Luminol/Enhancer Solution, 80mL
Stable Peroxide Solution, 80mL
Hybridization Buffer, 125mL
(2X) Hybridization Stringency Wash Buffer, 375mL
Blocking Buffer, 500mL
(4X) Wash Buffer, 500mL
Substrate Equilibration Buffer, 500mL
5X Heptanucleotide Mix, 100μL
5X Deoxynucleotide Mix, 80μL
10X Reaction Buffer, 50μL
Klenow DNA Polymerase, 10μL
Control DNA, 5μL
5 M Ammonium Acetate, 1mL
0.5 M EDTA, pH 8.0, 1mL
Nuclease-free Water, 1mL
Biotin-11-UTP, 20μL
No. of Reactions 10 labeling reactions and 10 mini-gel blots
Product Type North2South Complete Biotin Random Prime Labeling and Detection Kit
Quantity 2164mL kit

Thermo Scientific™ North2South Chemiluminescent Detection Kit combines a novel ECL substrate for horseradish peroxidase (HRP) and optimized hybridization and blocking buffers to ensure consistent, high-sensitivity Southern blot results.

The Thermo Scientific North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin-HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera.

Highlights:

  • Sensitivity equal to or greater than 32P
  • Amenable to many formats
  • High level of light output and consistent low background allows for very short exposure times using cooled CCD camera systems or film
  • Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user