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Nanog Rat anti-Mouse, eFluor 660, Clone: eBioMLC-51, eBioscience™

Rat Monoclonal Antibody

$216.05 - $549.55

Specifications

Antigen Nanog
Clone eBioMLC-51
Host Species Rat
Species Reactivity Mouse
Applications Flow Cytometry (Intracellular Staining)
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 Disclaimers

For Research Use Only.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

501124395

 
affymetrix
50576180
25μg Each for $216.05

501124396

 
affymetrix
50576182
100μg Each for $549.55
Description & Specifications

Specifications

Antigen Nanog
Clone eBioMLC-51
Host Species Rat
Species Reactivity Mouse
Applications Flow Cytometry (Intracellular Staining)
Applications Immunocytochemistry
Applications Immunohistochemistry
Applications Microscopy
Applications Western Blotting
Regulatory Status RUO
Conjugate eFluor 660
Format Conjugated
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 0.2mg/mL

The eBioMLC51 monoclonal antibody recognizes mouse Nanog. Nanog is a multidomain homeobox transcription factor that has been shown to maintain pluripotency of embryonic stem cells, independent of LIF/Stat3. Expression of Nanog in the mouse is specific to early embryos, the ICM of the blastocyst, embryonic stem (ES) cells, and embryonic germ (EG) cells. Nanog expression often overlaps, but is not identical to, that of Oct4. Nanog is downregulated upon cellular differentiation and loss of pluripotency, making it a suitable marker in determining the undifferentiated state of stem cells.

Nanog acts as a transcriptional activator and has two activation domains in the C-terminus, called CD2 and WR, and one activation domain in the N terminus. The CD2 domain is unique to Nanog, whereas the NK2 DNA binding domain is well conserved. Immunoblotting using eBioMLC51 reveals a band at ∽ 45kDa in F9 (an embryonal carcinoma cell line) lysate, but not in lysate from the NIH3T3 cell line or mouse spleen. Preliminary data using fluorochrome-conjugated eBioMLC51 suggests that it is essential to use the eBioscience Foxp3 Staining Buffer Set, Cat. 00-5523, for intracellular staining of mouse Nanog.