Myosin 4, Alexa Fluor® 488, Clone: MF20, eBioscience™
Mouse Monoclonal Antibody
Manufacturer: Life Technologies LS53650380
Description: This MF20 monoclonal antibody recognizes the heavy chain of myosin II, specificially the light meromyosin portion, in cardiac and skeletal muscle of vertebrates. Myosin II is composed of two heavy chains and four light chains. The 220-kDa myosin heavy chain exists as four different isoforms due to alternative splicing. Myosins interact with actin and hydrolyze ATP to function in muscle contraction, cytokinesis, and phagocytosis. The MF20 has been shown to react to myosin from a variety of mammalian, avian and amphibian species, including rat, mouse, human, chicken, zebrafish, and dog. Applications Reported: This MF20 antibody has been reported for use in immunohistochemical staining, immunocytochemistry, and immunohistochemical staining of frozen tissue sections. Applications Tested: This MF20 antibody has been tested by immunocytochemistry on fixed and permeabilized C2C12 cells that were differentiated for 2 days in low serum medium prior to staining. Staining can be performed using less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.Muscle contraction. Weiss A., J. Mol. Biol. 290:61-75(1999). Denis N.J., Proteomics 7:868-874(2007).
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Q076A5, Q076A6, P12882, Q9Y623, Q5SX39, Q5SX40, Q29RW1, Q28641, Q9BE40|
|MYH2B, MyHC-2B, MyHC-IIb|
|Bovine, Rat, Canine, Chicken, Chimpanzee, Feline, Guinea Pig, Human, Mouse, Non-human primate, Rabbit|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)|
|Alexa Fluor® 488|
|4° C, store in dark, DO NOT FREEZE!|
|100009548, 100125991, 100135061, 100726456, 17879, 17884, 281337, 287408, 360543, 408020, 4619, 4622, 468396, 479502|
For Research Use Only.