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Myosin 4, Alexa Fluor® 488, Clone: MF20, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS53650382

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Catalog No. 501128865



Description

Description

Description: This MF20 monoclonal antibody recognizes the heavy chain of myosin II, specificially the light meromyosin portion, in cardiac and skeletal muscle of vertebrates. Myosin II is composed of two heavy chains and four light chains. The 220-kDa myosin heavy chain exists as four different isoforms due to alternative splicing. Myosins interact with actin and hydrolyze ATP to function in muscle contraction, cytokinesis, and phagocytosis. The MF20 has been shown to react to myosin from a variety of mammalian, avian and amphibian species, including rat, mouse, human, chicken, zebrafish, and dog. Applications Reported: This MF20 antibody has been reported for use in immunohistochemical staining, immunocytochemistry, and immunohistochemical staining of frozen tissue sections. Applications Tested: This MF20 antibody has been tested by immunocytochemistry on fixed and permeabilized C2C12 cells that were differentiated for 2 days in low serum medium prior to staining. Staining can be performed using less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Muscle contraction. Weiss A., J. Mol. Biol. 290:61-75(1999). Denis N.J., Proteomics 7:868-874(2007).
Specifications

Specifications

Myosin 4
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q9BE40, Q28641, Q29RW1, Q5SX40, Q5SX39, Q9Y623, P12882, Q076A6, Q076A5
MYH2B, MyHC-2B, MyHC-IIb
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Bovine, Rat, Canine, Chicken, Chimpanzee, Feline, Guinea Pig, Human, Mouse, Non-human primate, Rabbit
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
MF20
Alexa Fluor® 488
MYH1
Liquid
MYH1, MYH4
IgG2b, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
100009548, 100125991, 100135061, 100726456, 17879, 17884, 281337, 287408, 360543, 408020, 4619, 4622, 468396, 479502
Documents

For Research Use Only.