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MP Biomedicals™ Trypsin, Porcine Pancreas

Typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.

$222.34 - $222.34

Specifications

Product Type Trypsin
Format Powder
For Use With (Application) Release adherent cells from tissue culture plates for passaging
Sample Type Porcine
Storage Requirements 4°C
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Products 1
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
ICN15357125 View Documents MP Biomedicals™
0215357125*
25g Each for $222.34
 
Description

Description

  • CAS Number: 9002-07-7
  • Molecular Weight: Approximately 23,000 or 22000 (for human)
  • Composition: Trypsin is composed of two subunits, a-trypsin and b-trypsin. a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain.
  • Activity: One BAEE unit will produce a DA253 of 0.001 per minute at pH 7.6 at 25°C using BAEE as substrate. Reaction volume = 3.2mL (1 cm light path).
    One TAME unit hydrolyzes 1 umole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25°C, pH 8.2, in the presence of 0.001 M calcium ion.
    One USP trypsin unit is the activity causing a change in absorbance of 0.003 per minute under the conditions specified.
  • Activity Conversion:1 TAME unit = 19.2 USP or NF units = 57.5 BAEE Units
  • Composition: Trypsin is composed of two subunits, a-trypsin and b-trypsin. a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain.
Specifications

Specifications

Trypsin
Release adherent cells from tissue culture plates for passaging
4°C
Powder
Porcine