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MP Biomedicals™ Proteinase K from Tritirachium album

Highly active stable endopeptidase with a broad spectrum of action

$66.58 - $453.58

Specifications

Product Type Proteinase K
Format Lyophilized Powder
Grade Molecular biology reagent
Inhibitors Diisopropylfluorophosphate or Phenylmethane Sulfonyl Fluoride, AEBSF and Trypsin
Molecular Weight 30000
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Products 5
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
ICN19398125
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MP Biomedicals™
0219398125
25mg
Each for $100.62
 
ICN19398110
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MP Biomedicals™
0219398110
10mg
Each for $81.51
 
ICN19398105
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MP Biomedicals™
0219398105
5mg
Each for $66.58
 
ICN19398180
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MP Biomedicals™
0219398180
100mg
Each for $182.32
 
ICN19398190
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MP Biomedicals™
0219398190
500mg
Each for $453.58
 
Description

Description

Proteinase K is used in digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA, as well as in removal of endotoxins bound to cationic proteins such as lysozyme and ribonuclease A and in determination of enzyme localization on membranes.

  • Grade: Molecular biology reagent
  • Specific Activity: >30 mAnson u/mg
  • Presentation: White powder
  • Isoelectric Point: 8.9 (Lit.)
  • Extinction Coefficient: E1%= 14.2 (280 nm,10 mM NaCl and 5 mM CaCl2, pH 8.0) (Lit.)
  • pH: 7.5 -12.00 (denatured hemoglobin as substrate) (Lit.)
  • Soluble in water

One Anson unit is the amount of enzyme which will liberate 1 °mole of folin-positive amino acids per minnute at pH 7.5 and 35°C using hemoglobin as substrat

Isolation of DNA and RNA

Specifications

Specifications

Proteinase K
Molecular biology reagent
30000
Tritirachium album
Lyophilized Powder
Diisopropylfluorophosphate or Phenylmethane Sulfonyl Fluoride, AEBSF and Trypsin
7.5 to 12
4°C
SDS