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MP Biomedicals Proteinase K from Tritirachium album

Highly active stable endopeptidase with a broad spectrum of action

$61.74 - $1,521.15

Specifications

Product Name Proteinase K
For Use With (Application) Useful for the proteolytic inactivation of Nucleases during the isolation of DNA and RNA
Grade Molecular biology reagent
Inhibitors Diisopropylfluorophosphate or Phenylmethane Sulfonyl Fluoride, AEBSF and Trypsin
Molecular Weight 30000
View More Specs
Products
Catalog Number Mfr. No. Quantity Price Quantity    

ICN19398125

 
mp biomedicals
0219398125
25mg Each for $93.30

ICN19398110

 
mp biomedicals
0219398110
10mg Each for $75.49

ICN19398105

 
mp biomedicals
0219398105
5mg Each for $61.74

ICN19398180

 
mp biomedicals
0219398180
100mg Each for $183.22

ICN19398190

 
mp biomedicals
0219398190
500mg Each for $822.92

ICN19398191

 
mp biomedicals
0219398191
1g Each for $1,521.15
Description & Specifications

Specifications

Product Name Proteinase K
For Use With (Application) Useful for the proteolytic inactivation of Nucleases during the isolation of DNA and RNA
Grade Molecular biology reagent
Inhibitors Diisopropylfluorophosphate or Phenylmethane Sulfonyl Fluoride, AEBSF and Trypsin
Molecular Weight 30000
pH 7.5 to 12
Source Tritirachium album
Storage Requirements 4°C

Proteinase K is used in digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA, as well as in removal of endotoxins bound to cationic proteins such as lysozyme and ribonuclease A and in determination of enzyme localization on membranes.

  • Grade: Molecular biology reagent
  • Specific Activity: >30 mAnson u/mg
  • Presentation: White powder
  • Isoelectric Point: 8.9 (Lit.)
  • Extinction Coefficient: E1%= 14.2 (280 nm,10 mM NaCl and 5 mM CaCl2, pH 8.0) (Lit.)
  • pH: 7.5 -12.00 (denatured hemoglobin as substrate) (Lit.)
  • Soluble in water

One Anson unit is the amount of enzyme which will liberate 1 °mole of folin-positive amino acids per minnute at pH 7.5 and 35°C using hemoglobin as substrat

Isolation of DNA and RNA