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MP Biomedicals Proteinase K from Tritirachium album

Highly active stable endopeptidase with a broad spectrum of action

$59.70 - $1,471.30

Specifications

Product Name Proteinase K
For Use With (Application) Useful for the proteolytic inactivation of Nucleases during the isolation of DNA and RNA
Grade Molecular biology reagent
Inhibitors Diisopropylfluorophosphate or Phenylmethane Sulfonyl Fluoride, AEBSF and Trypsin
Molecular Weight 30000
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Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
ICN19398125 MP Biomedicals
19398125
25mg Each for $90.27
ICN19398110 MP Biomedicals
19398110
10mg Each for $73.10
ICN19398105 MP Biomedicals
19398105
5mg Each for $59.70
ICN19398180 MP Biomedicals
19398180
100mg Each for $177.23
ICN19398190 MP Biomedicals
19398190
500mg Each for $796.41
ICN19398191 MP Biomedicals
19398191
1g Each for $1,471.30
Specifications

Description

Proteinase K is used in digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA, as well as in removal of endotoxins bound to cationic proteins such as lysozyme and ribonuclease A and in determination of enzyme localization on membranes.

  • Grade: Molecular biology reagent
  • Specific Activity: >30 mAnson u/mg
  • Presentation: White powder
  • Isoelectric Point: 8.9 (Lit.)
  • Extinction Coefficient: E1%= 14.2 (280 nm,10 mM NaCl and 5 mM CaCl2, pH 8.0) (Lit.)
  • pH: 7.5 -12.00 (denatured hemoglobin as substrate) (Lit.)
  • Soluble in water

One Anson unit is the amount of enzyme which will liberate 1 °mole of folin-positive amino acids per minnute at pH 7.5 and 35°C using hemoglobin as substrat

Isolation of DNA and RNA

Description & Specifications

Specifications

Proteinase K
Molecular biology reagent
30000
Tritirachium album
Useful for the proteolytic inactivation of Nucleases during the isolation of DNA and RNA
Diisopropylfluorophosphate or Phenylmethane Sulfonyl Fluoride, AEBSF and Trypsin
7.5 to 12
4°C