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MP Biomedicals™ Proteinase K from Tritirachium album

Highly active stable endopeptidase with a broad spectrum of action

Manufacturer:  MP Biomedicals™ 0219398105

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Catalog No. ICN19398105



Proteinase K is used in digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA, as well as in removal of endotoxins bound to cationic proteins such as lysozyme and ribonuclease A and in determination of enzyme localization on membranes.

  • Grade: Molecular biology reagent
  • Specific Activity: >30 mAnson u/mg
  • Presentation: White powder
  • Isoelectric Point: 8.9 (Lit.)
  • Extinction Coefficient: E1%= 14.2 (280 nm,10 mM NaCl and 5 mM CaCl2, pH 8.0) (Lit.)
  • pH: 7.5 -12.00 (denatured hemoglobin as substrate) (Lit.)
  • Soluble in water

One Anson unit is the amount of enzyme which will liberate 1 °mole of folin-positive amino acids per minnute at pH 7.5 and 35°C using hemoglobin as substrat

Isolation of DNA and RNA



5 mg
Diisopropylfluorophosphate or Phenylmethane Sulfonyl Fluoride, AEBSF and Trypsin
Proteinase K
7.5 to 12
Tritirachium album
Lyophilized Powder
Molecular Biology
Useful for the proteolytic inactivation of Nucleases during the isolation of DNA and RNA
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