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Human VEGF-165 Animal Free, Recombinant Protein, eBioscience™
Manufacturer: Life Technologies LS68878463
DescriptionThe vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells. Description: This SAFE™ (Serum and Animal Free, low Endotoxin) protein has been made in plants with no exposure to animal protein during expression or purification. This animal free product has very low levels of endotoxin. Vascular endothelial growth factor (VEGF), also called vascular permeability factor (VPF), is produced by cultured vascular smooth muscle cells. From analysis of transcripts from these cells by PCR and cDNA cloning, 3 different forms of the VEGF coding region have been identified. These cDNAs had predicted products of 189, 165, and 121 amino acids. VEGF is a mitogen primarily for vascular endothelial cells. It is structurally related to platelet-derived growth factor. VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. The VEGF receptor, Flk1, is exclusively expressed in endothelial cells. VEGF may be a major regulator of tumor angiogenesis in vivo. VEGF165 is the most common form in most tissues. Applications Reported: SAFE™ Human VEGF165 Recombinant Animal Free Protein is biologically active. Applications Tested: The ED50 of this protein, as measured by cell proliferation of HUVECS (human umbilical vein endothelial cells), is < 10ng/mL. This corresponds to a specific activity of greater than or equal to 1 x 10e5 Units/mg. Source: Plant expressed 165 amino acids (Ala27-Arg191) with 16 amino acid Histidine tag. accession NP_001165097. Bioactivity: The ED50 of this protein, as measured by cell proliferation of HUVECS (human umbilical vein endothelial cells), is <10ng/mL. This corresponds to a specific activity of greater than or equal to 1 x 10e5 Units/mg. Endotoxin: Less than 0.005ng/ug (0.05 EU/ug) as measured by turbidity assay. Purity: Greater than or equal to 95% as determined by SDS-PAGE. Molecular Weight: 21.3kDa (with glycosylation the apparent molecular weight is 30 kDa). Storage: The lyophilized powder can be stored at less than or equal to -20°C. (Short term storage at 2-8°C). Reconstitute in sterile reagent grade deionized water to a concentration no less than 100 ug/mL. Once reconstituted, protein should be aliquoted and stored at -20°C or lower. For best stability add 0.1% BSA before freezing aliquots.
|Human VEGF-165 Animal Free|
|4°C or -20°C if preferred|
|Plant expressed 165 amino acids (Ala27-Arg191) with 16 amino acid Histidine tag. Accession NP_001165097|
|Less than 0.005ng/μg (0.05 EU/μg) as measured by turbitidity assay|
|The ED50 of this protein, as measured by cell proliferation of HUVECS (human umbilical vein endothelial cells), is <10ng/mL. This corresponds to a specific activity of greater than or equal to 1 x 105 Units/mg.|
|PBS with no preservative; pH 7.2|
|21.3kDa (with glycosylation the apparent molecular weight is 30kDa)|
|Human VEGF-165 Animal Free|
|Control, Functional Assay|