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eBioscience™ Mouse TNF alpha, Recombinant Protein

Mouse Recombinant Protein

$129.05 - $274.05

Specifications

Structural Form E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF- alpha accession # NM_013693
Purity >98%
Purification Method SDS-PAGE
Molecular Weight The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17kDa polypeptides on SDS-PAGE.
Source E. coli
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 Disclaimers

For Research Use Only.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

501128912

 
ebioscience™
14-8321-62
5μg Each for $129.05

501128929

 
ebioscience™
14-8321-63
10μg Each for $274.05
Description & Specifications

Specifications

Structural Form E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF- alpha accession # NM_013693
Purity >98%
Purification Method SDS-PAGE
Molecular Weight The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17kDa polypeptides on SDS-PAGE.
Source E. coli
Accession Number NM_013693
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay.
Formulation Sterile liquid; 10mM sodium phosphate, pH 7.2, 150mM NaCl, 0.5% BSA. 0.22μm filtered.
Biological Activity The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 107 Units/mg.
For Use With (Application) Cytokine Bioassays
Validated Application FA
Conjugate Unlabeled
Regulatory Status RUO
Cross Reactivity Mouse
Gene Symbol Tumor Necrosis Factor alpha, TNFa TNF-a
Name TNF alpha
Recombinant Yes

Mouse TNF-a is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF-α is expressed as a membrane-bound molecule. TNF-α is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF-α have been observed.