missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

Thermo Scientific™ Pierce™ Mouse TNF alpha ELISA Kits

Measure levels of mouse tumor necrosis factor alpha (TNFa) in mouse serum and cell culture supernatant samples using 96-well plates.

$651.05 - $2,436.00

Specifications

Format Colorimetric ELISA (TMB substrate) in clear 96 well strip plate
Measurement Absorbance at 450nm minus absorbance at 550nm
Precision Intra-assay CV <10%; inter-assay CV <10%
Sample Type Cell culture supernatant, serum
Target Natural and recombinant mouse TNFa (mouse tumor necrosis factor alpha)
View More Specs


Products ${productFamilyLength}
Catalog Number Mfr. No. Sufficient For Price Quantity  
Catalog Number Mfr. No. Sufficient For Price Quantity  
ENEMTNFA Thermo Scientific™ Pierce™
EMTNFA
96 microplate wells Each for $651.05
ENEMTNFA2 Thermo Scientific™ Pierce™
EMTNFA2
2 x 96 microplate wells Each for $1,145.50
ENEMTNFA5 Thermo Scientific™ Pierce™
EMTNFA5
5 x 96 microplate wells Each for $2,436.00
Specifications

Description

Assay TNFα (mouse tumor necrosis factor alpha) levels in mouse serum and culture supernatant samples with our Thermo Scientific™ Mouse TNF alpha ELISA Kits.

These mouse TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched TNFα antibody pairs and TNFα standard only. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant mouse TNFα (mouse tumor necrosis factor alpha)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96 well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 50 to 2450pg/mL

  • Sensitivity: < 9pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, IFNα or human TNFα. This ELISA has some cross-reactivity with rabbit and cynomologous monkey TNFα in culture supernatants

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

Description & Specifications

Specifications

Colorimetric ELISA (TMB substrate) in clear 96 well strip plate
Intra-assay CV <10%; inter-assay CV <10%
Natural and recombinant mouse TNFa (mouse tumor necrosis factor alpha)
Absorbance at 450nm minus absorbance at 550nm
Cell culture supernatant, serum