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Thermo Scientific™ Pierce™ Mouse TNF alpha ELISA Kits


Measure levels of mouse tumor necrosis factor alpha (TNFa) in mouse serum and cell culture supernatant samples using 96-well plates.

Manufacturer: thermo scientific™ pierce™  EMTNFA

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Catalog No. ENEMTNFA

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Description & Specifications

Specifications

Precision Intra-assay CV <10%; inter-assay CV <10%
Sensitivity <9pg/mL
Description Mouse TNF alpha ELISA Kit
Kit Contents 30X Wash Buffer, 50mL
Sample Type Cell culture supernatant, serum
Measurement Absorbance at 450nm minus absorbance at 550nm
Sufficient For 96 microplate wells
Sample Size 50µL
Total Assay Time 3 hr.
Specificity No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, IFNa or human TNFa. This ELISA has some cross-reactivity with rabbit and cynomologous monkey TNFa in culture supernatants
Formulation Complete ELISA kit with coated 96-well plate
Format Colorimetric ELISA (TMB substrate) in clear 96 well strip plate
Target Natural and recombinant mouse TNFa (mouse tumor necrosis factor alpha)
Assay Range 50 to 2450pg/mL
Quantity 1 plate kit

Assay TNFα (mouse tumor necrosis factor alpha) levels in mouse serum and culture supernatant samples with our Thermo Scientific™ Mouse TNF alpha ELISA Kits.

These mouse TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched TNFα antibody pairs and TNFα standard only. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant mouse TNFα (mouse tumor necrosis factor alpha)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96 well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 50 to 2450pg/mL

  • Sensitivity: < 9pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, IFNα or human TNFα. This ELISA has some cross-reactivity with rabbit and cynomologous monkey TNFα in culture supernatants

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).