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Thermo Scientific™ Pierce™ Mouse TNF alpha ELISA Kits

Measure levels of mouse tumor necrosis factor alpha (TNFa) in mouse serum and cell culture supernatant samples using 96-well plates.

Manufacturer:  Thermo Scientific™ Pierce™ EMTNFA

 View more versions of this product

Catalog No. ENEMTNFA


 

Specifications

Description

Assay TNFα (mouse tumor necrosis factor alpha) levels in mouse serum and culture supernatant samples with our Thermo Scientific™ Mouse TNF alpha ELISA Kits.

These mouse TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched TNFα antibody pairs and TNFα standard only. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant mouse TNFα (mouse tumor necrosis factor alpha)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96 well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 50 to 2450pg/mL

  • Sensitivity: < 9pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, IFNα or human TNFα. This ELISA has some cross-reactivity with rabbit and cynomologous monkey TNFα in culture supernatants

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

Description & Specifications

Specifications

Intra-assay CV <10%; inter-assay CV <10%
Mouse TNF alpha ELISA Kit
Cell culture supernatant, serum
96 microplate wells
3 hr.
Complete ELISA kit with coated 96-well plate
Natural and recombinant mouse TNFa (mouse tumor necrosis factor alpha)
1 plate kit
<9pg/mL
30X Wash Buffer, 50mL
Absorbance at 450nm minus absorbance at 550nm
50µL
No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, IFNa or human TNFa. This ELISA has some cross-reactivity with rabbit and cynomologous monkey TNFa in culture supernatants
Colorimetric ELISA (TMB substrate) in clear 96 well strip plate
50 to 2450pg/mL