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Mouse TGF beta 1 Carrier-Free, Recombinant Protein, eBioscience™

Recombinant Protein

Manufacturer:  Life Technologies LS34834282

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Catalog No. 501129280



Description

Description

TGF beta-1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its prodomain. Activation of TGF-b1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF-beta, an aminoterminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. Description: Mouse TGF beta 1 (Transforming Growth Factor beta 1) is a member of a superfamily of homologous, disulfide-linked, homodimeric proteins that regulate the proliferation and differentiation of normal and transformed cells. Human TGF beta 1 is a 25kDa protein with each subunit containing 112 aa residues. TGF beta 1 is secreted from the many cell types in a complex with LAP (Latency Associated Peptide) and also with LTBP (latent TGF beta binding protein). Disassociation is thought to happen in the extracellular matrix via proteases and/or integrins. Regulatory T cells are a recently identified source of TGF beta 1 thought to be a key player in their suppressive functions. TGF beta 1 binds through TGF beta receptors (of which there is a large family). The protein is highly conserved across all mammalian species. Applications Reported: Recombinant mouse TGF-β1 is biologically active and can inhibit IL-4 induced proliferation of mouse HT-2 cells. Applications Tested: The ED50 of this protein, as measured by inhibition of IL-4 induced proliferation in HT-2 cells, is less than or equal to 40 pg/ml. This corresponds to a specific activity of greater than or equal to 2.5 x 10e7 Units/mg. Source: TGFb1 was expressed in CHO cells. Mature TGFb1 (ala279-ser390; accession #BC001180) was purified from the conditioned media. Bioactivity: The ED50 of this protein, as measured by inhibition of IL-4 induced proliferation in HT-2 cells, is less than or equal to 40 pg/ml. This corresponds to a specific activity of greater than or equal to 2.5 x 10e7 units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >95% as determined by SDS-PAGE. Molecular Weight: Mature TGFb1 polypeptide has a predicted molecular mass of 12,794. On reducing SDS-PAGE the protein migrates as a 13kDa polypeptide and on non-reducing SDS page the cystine-linked homodimer migrates as a 25kDa protein. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Specifications

Specifications

P04202
Functional Assays, Cell Culture
protein with 0.1M glycine and no preservative; pH 3.0
Mature TGFb1 polypeptide has a predicted molecular mass of 12,794. On reducing SDS-PAGE the protein migrates as a 13kDa polypeptide and on non-reducing SDS page the cystine-linked homodimer migrates as a 25kDa protein.
3.0
Affinity chromatography
CHO Cells
RUO
Recombinant
Mouse TGF beta 1 Carrier-Free
Control, Functional Assay, Neutralization
Mouse
Ms
Recombinant
0.5mg/mL
Liquid
21803
Mouse TGF beta 1 Carrier-Free
SDS-PAGE
100μg
-80°C
TGFb1 was expressed in CHO cells.
Mature TGFb1 (ala279 - ser390; accession #BC001180) was
purified from the conditioned media.
Less than 0.01ng/μg cytokine as determined by the LAL assay.
Tgfb1
The ED50 of this protein, as measured by inhibition of IL-4 induced proliferation in HT-2 cells, is less than or equal to 40pg/mL. This corresponds to a specific activity of greater than or equal to 2.5 x 107 units/mg.
Unlabeled
CHO cells
Documents

For Research Use Only.