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Invitrogen™ eBioscience™ Mouse ST2 (IL-33R) ELISA Ready-SET-Go!™ Kit

Specifically engineered for accurate and precise measurement of mouse ST2 (IL-33R) protein levels from samples including serum and cell culture supernatants.

Manufacturer:  Invitrogen™ LS88933488

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Catalog No. 501125235


Alert:

Contains Sodium azide


Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant protein for generating standard curve and calibrating samples
Coating Buffer. 10X PBS ELISA Coating Buffer
Assay Diluent. 5X Concentrated
Sample Diluent B
Detection Enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards.

Description

Description

ST2 is a member of the interleukin 1 receptor family. Studies of ST2 in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. ST2 belongs to the interleukin-1 receptor family with three Ig-like C2-type (immunoglobulin-like) domains and a TIR domain. ST2 is a receptor for interleukin-33 (IL-33) which upon stimulation recruits the signaling molecules MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Reports also suggest its possible role in helper T-cell function as well as in cell growth. Three isoforms of ST2 have been reported where isoform C is a membrane form and isoform B is a secreted form. ST2 may be induced by proinflammatory stimuli, and may also be found in serum of autoimmune disease. Different isoforms are restricted to different tissues while high expression of this protein is evident in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine.
Specifications

Specifications

P14719
Biotin
17082
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Sample Diluent B: 12 mL of a 1X solution per plate, Detection Enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards., 96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86)
10 x 96 tests
80 pg/mL
Serum (50μL), Supernatants (50μL)
2-8°C
24 hr 30 min
Il1rl1
HRP
80-10,000 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA
RUO
Serum, Supernatants
Mouse
1 hr 45 min
Mouse
IL1RL1, Interleukin-1 Receptor-Like 1, Ly84
Documents

For Research Use Only