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Thermo Scientific™ Pierce™ Mouse Soluble ICAM-1 (CD54) ELISA Kits

Measure levels of mouse soluble ICAM-1 (CD54) in mouse serum, plasma (EDTA) and cell culture supernatant samples using 96-well plates.

Manufacturer:  Thermo Scientific™ Pierce™ EMICAM12

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Catalog No. ENEMICAM12


 

Specifications

Description

Assay sICAM-1 (mouse soluble ICAM-1, also called CD54) levels in mouse serum, plasma (EDTA) and culture supernatant samples with our Thermo Scientific Mouse Soluble ICAM (CD54) ELISA Kits.

These mouse sICAM-1 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of soluble ICAM-1 (intercellular adhesion molecule 1, also called CD54) in samples at 50μL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Mouse soluble ICAM-1 (intercellular adhesion molecule 1, also called CD54)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 25.6 to 1000ng/mL

  • Sensitivity: < 5ng/mL

  • Precision: Intra-assay CV < 10%; inter assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3.5 hours

  • Sample Size: 50μL of 100 fold diluted samples per well

  • Specificity: No significant cross reaction with rat or human sICAM 1

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Description & Specifications

Specifications

Intra-assay CV <10%; inter assay CV <10%
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Mouse Soluble ICAM-1 (CD54) ELISA Kit
30X Wash Buffer, 2 x 50mL
Mouse soluble ICAM-1 (intercellular adhesion molecule 1, also called CD54)
Complete ELISA kit with coated 96-well plate
3.5 hr.
96 microplate wells
<5pg/mL
No significant cross reaction with rat or human sICAM 1
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
2 plate kit
50µL of 100-fold diluted samples per well
25.6 to 1000pg/mL
Absorbance at 450nm minus absorbance at 550nm